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hos: (hypo-osmotic swelling test) take 500 micro litre hts-h. O. S. Solutions in a small test tube. Add 100 micro liter ofliquified semen sample to the h. O. S. Solutions and mix well. Keep at 37 degreecentigrade for 30 minutes. Add 50 mirco litre of stop solution and take just one drop on the plain glass slide putthe cover slip and examin under microscope in 40x objective, calculate percentage ofcoiled tail.